Nexus Research Group - Rhizobium in New Zealand

The Expression in Soil Bacreria of Symbiotic Genes from Rhizobium Leguminosarum biovar trifolii

By Michael Fenton (1994)

Dept of Microbiology and Genetics, Massey University, Palmerston North, NEW ZEALAND

Nodule Formation on White Clover Seedlings Thirty Days After Inoculation

Left to right:

Water control (lacks root nodules)
Rhizobium strain 2163::Tn5 (nodules present)
Sphingobacterium NZRM1228 (lacks root nodules)
Transconjugant MF100 (NZRM1228 containing Tn5 marked symbiotic plasmid from strain 2163::Tn5)

Note root nodules present on plant (4) but the transconjugant Sphingobacterium multivorum inside are unable to fix nitrogen.

Left to right:

Rhizobium strain 2163::Tn5
Transconjugant MF100

Note root nodules present on plant (2) are greater in number but the transconjugant Sphingobacterium multivorum inside are unable to fix nitrogen.The plant is no bigger than the water control plant.

(Reproduced with permission)

The Expression in Soil Bacreria of Symbiotic Genes from Rhizobium Leguminosarum biovar trifolii

Nodule Formation on White Clover Seedlings Thirty Days After Inoculation

RETURN TO... Symbiotic plasmid transfer in Rhizobium sp.